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引用:鞠雅晗*,汤志敏*,王宇瑶,代小婵,罗敏,谷平.不同波长的蓝光对人视网膜色素上皮细胞的影响.国际眼科杂志 2020;20(8):1315-1319,doi:10.3980/j.issn.1672-5123.2020.8.04
不同波长的蓝光对人视网膜色素上皮细胞的影响
Effects of different wavelengths of blue light on human retinal pigment epithelial cells
投稿时间:2019-11-25  修订日期:2020-07-01
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DOI:10.3980/j.issn.1672-5123.2020.8.04
关键词:  蓝光  视网膜色素上皮细胞  增殖  视循环  炎症
Key Words:  blue light  retinal pigment epithelial cell  proliferation  visual cycle  inflammation
基金项目:国家自然科学基金项目(No.81870687)
Fund Project:National Natural Science Foundation of China(No.81870687)
                 
作者单位
鞠雅晗* 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
汤志敏* 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
王宇瑶 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
代小婵 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
罗敏 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
谷平 中国上海市,上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室*:鞠雅晗和汤志敏对本文贡献一致。
                 
AuthorInstitution
Ya-Han Ju* Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
Zhi-Min Tang* Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
Yu-Yao Wang Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
Xiao-Chan Dai Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
Min Luo Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
Ping Gu Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai , China; Co-first authors: Ya-Han Ju and Zhi-Min Tang
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摘要:
      

目的:探讨不同波长的蓝光对人视网膜色素上皮细胞(RPE)的影响。

     方法:将体外培养的ARPE-19细胞随机分为对照组、447nm蓝光组、456nm蓝光组、468nm蓝光组,对照组细胞于常规条件下培养,蓝光组细胞使用光强为200Lx的OLED蓝光背光源照射72h,利用细胞活/死染色实验、CCK-8实验、Real-time PCR等方法比较不同波长的蓝光对细胞形态、细胞活性、增殖能力及视循环功能指标和炎症指标mRNA表达的影响。

     结果:蓝光照射后,ARPE-19细胞的形态发生变化,细胞融合减少。蓝光波长越短,对细胞增殖抑制作用越明显,细胞内增殖标志物Ki-67 mRNA表达越少,视循环功能指标卵磷脂视黄醇酰基转移酶(LRAT)、视黄醛结合蛋白(CRALBP)、视黄醛脱氢酶(RDH)、光受体视黄醇类结合蛋白(IRBP)mRNA表达下调越明显,细胞内炎症因子单核细胞趋化因子(MCP-1)、白介素-6(IL-6)mRNA表达水平上调越明显。

     结论:不同波长蓝光对RPE细胞均有损害作用,且蓝光波长越短,其损害作用越大。

Abstract:
      AIM: To investigate the effects of different wavelength of blue light on human retinal pigment epithelial(RPE)cells.

     METHODS: ARPE-19 cells cultured in vitro were randomly divided into four groups, which were control group, 447nm blue light group, 456nm blue light group and 468nm blue light group. The cells in control group were cultured under normal conditions whereas the cells in blue light group were irradiated with different wavelengths of OLED blue light with the illumination intensity of 200Lux for 72h. Live/Dead staining assay, CCK-8 assay and real-time PCR were performed to compare the effects of different wavelengths of blue light on the morphology, cell viability, proliferation capacity, mRNA expression level of visual cycle biomarkers and inflammatory biomarkers of ARPE-19 cells, respectively.

     RESULTS: After blue light irradiation, the abnormal morphology and the decrease of cell confluence of ARPE-19 cells were observed. Furthermore, with the decrease in the wavelength of blue light, the inhibition effect of blue light on RPE proliferation was enhanced, and the mRNA expression of the proliferation marker Ki-67 and visual cycle biomarkers LRAT, CRALBP, RDH and IRBP decreased. In addition, the mRNA expression levels of inflammatory factors MCP-1 and IL-6 in RPE cells were up-regulated with the decrease in the wavelength of blue light.

     CONCLUSION: Our data demonstrated that blue light in different wavelengths exerted detrimental effects on RPE cells. The shorter the wavelength of blue light was, the more severe damage it caused on the RPE cells.

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